automacs running buffer (Miltenyi Biotec)

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Miltenyi Biotec automacs running buffer
Automacs Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/running+buffer/autoMACS+Running+Buffer+-+MACS+Separation+Buffer/us12692546-503-17-20
Average 97 stars, based on 371 article reviews

automacs running buffer - by Bioz Stars, 2026-09

97/100 stars

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Incubation:

Article Title: Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics.
Article Snippet: MACSima multiplexed staining and imaging The MACSima Imaging System by Miltenyi Biotec Biotec is a fully automated instrument seamlessly integrating liquid handling with widefield microscopy for cyclic immunofluorescence imaging.21 Before initiating the MACSima instrument, a DAPI pre-staining step was performed. .. The running buffer was removed from the sample, which was then incubated for 10 minutes with a 1:10 dilution of DAPI staining solution (Miltenyi Biotec 130-127-574). .. After staining, the sample underwent three washing steps with running buffer (Miltenyi Biotec 130-121-565), followed by the addition of 550 mL of running buffer.

Staining:

Article Title: Compositions and methods for treating cancer with anti-CD19/CD22 immunotherapy
Article Snippet: .. Cells were washed and stained with cold AutoMACS buffer supplemented with 0.5% bovine serum albumin (Miltenyi Biotec), and resuspended in 200 ul Running Buffer before acquired by MACSQuant®10 Analyzer (Miltenyi Biotec). .. For CAR surface expression, CD22-his peptide (Abcam, Cambridge, MA) and CD19Fc peptide (R&D System, Minneapolis, MN) were used followed by anti His-PE or anti Fc-AF647 (Jackson ImmunoResearch, West Grove, PA).

Article Title: Differential CD8+ T/NK cell-mediated reduction of HIV-1 replication after combination of ART with TIGIT or KLRG1 blockade in humanized mice
Article Snippet: Different antibody cocktails containing mAbs identifying NK and CD3+ T cells from PWH PBMCs were tested by FACS using a cell viability marker as Viability Dye (Ghost DyeTM, Invitrogen) incubated in PBS for 10 min at 4°C in ice. .. PBMCs were stained with superficial antibodies, anti-CD56, anti-CD16, anti-CD3 and anti-human IgG antibodies (BioLegend) in Running Buffer (RB; Miltenyi Biotec; containing BSA, EDTA and 0.09% azide) for 15 min at 4°C in ice. .. After incubation, cells were washed and subsequently, cells were stained with an FITC-conjugated anti-human IgG secondary antibody (1:200 dilution; BioLegend), directed to the heavy chains of primary antibodies, for 20 min at 4°C in ice in RB.

other:

Article Title: A pathological role of O-GlcNAcylation-driven TR11B production and function in lung adenocarcinoma.
Article Snippet: Article

Isolation:

Article Title: The diverse N -glycosylation profiles of CD4 + CD25 - and CD4 + CD25 + T cells in Hashimoto’s thyroiditis
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from 20 mL of whole blood, which was diluted into 35 mL Running Buffer (PBS pH 7.2 with BSA, EDTA, and 0.09% azide) (130-091-221, Miltenyi Biotec, Teterow, Germany). .. Diluted blood was layered on 15 mL Histopaque-1077 (10771, Sigma-Aldrich, Saint Louis, MO, USA) and centrifuged at 1500 rpm for 25 min at room temperature (RT).

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